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But as his mother was only a minor wife, his marriage to Hatshepsut (also a daughter of Thutmose I, by his principal wife Ahmose) cemented his position in line to the throne. Around 500 years ...
In addition to Hatshepsut—and another younger daughter who apparently died in childhood—it’s believed that Thutmose I fathered two sons with Queen Ahmes, both of whom predeceased him.