Competitive PCR–ELISA protocols for the quantitative and the standardized detection of viral genomes
Competitive PCR–ELISA combines competitive PCR with an ELISA to allow quantitative detection of PCR products. It is based on the inclusion of an internal standard competitor molecule that is designed ...
Discover the latest articles and news in related subjects. As an alternative to the separation PCR step, LN1- and LN2-ligated plasmid DNA were mixed in different proportions and used in a ...
Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...
A new diagnostic tool promises to sharpen the way scientists track two closely related blood parasites of wild canids.
The evolution of PCR technology is shaping the future of diagnostics, especially in viral detection and RNA analysis. In the second episode of our New Frontiers in PCR Technology Series, we have ...
A newly validated droplet digital PCR assay detected Onchocerca lupi infections in dogs and cats that conventional and ...
What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...
In the nearly two decades since its discovery, the polymerase chain reaction (PCR) and its derivative techniques have revolutionized molecular biology. The exponential nature of the technique allows ...
Even if you know the name "PCR test"...With the COVID-19 pandemic, the term "PCR test" has become completely familiar.However ...
In the six decades since Watson and Crick identified the double helical structure of DNA, a series of elegant and powerful experiments has established the broad outlines of how genetic information is ...
Quantitative polymerase chain reaction, or "qPCR", is considered the “second generation” of PCR and was first described in 1992. The past three decades have seen significant advances in qPCR ...
SalivaDirect, an open-source protocol, avoids many of the supply bottlenecks of other tools and could be offered for as little as $10 a test. From GLP-1 pioneers to researchers who uncovered the ...
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