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Proteins were separated on non-reducing 4–12% Tris-glycine or 4–12% Bis-Tris gradient gels before blotting or mass spectrometry. To examine E2 charging in vivo, cells were lysed in 50 mM MES ...
A 10% Bis-Tris gel of 1.0 mm thickness is prepared. To obtain a clear and more effective resolution of histone proteins, a higher percentage gel (15%) is proposed. The histone samples are loaded ...
Unlike the typical Tris-Glycine-based gels, the mPAGE® Lux Casting System employs the pH-neutral Bis-Tris in their gel solutions, which allows researchers to obtain sharper bands and reduces the gel’s ...
This innovative system accelerates gel curing through ultraviolet irradiation and Bis-Tris gel chemistry, ensuring consistent results while saving time and resources. Fewer steps reduce the chances of ...