Bacterial pellets were boiled for 10 minutes at 100°C in reducing conditions (1x 1,4-dithioreitol, Sigma Aldrich 15508013) and equilibrated in LDS sample buffer 1x (ThermoFisher J61894) and 10 µl were ...

Cell media, concentrated EV solution, and cell lysate were diluted in 1X reducing Laemmli SDS sample buffer (ThermoScientific), heated at 95°C for 10 min, and loaded on NuPAGE 12%, Bis–Tris gel ...

The purified antibodies were analyzed by SDS-PAGE and native polyacrylamide gel electrophoresis on NuPAGE 4–12% Bis-Tris gels (Thermo Fisher Scientific MS, USA). The binding kinetics and affinity of ...

Equal quantities of lysates were electrophoresed on a 4% to 12% gradient Bis-Tris midi gel followed by transfer onto polyvinylidene difluoride (PVDF) membrane using iBlot2 PVDF transfer stacks.

1/10 volume of 5% Coomassie blue G-250 (Invitrogen BN2004) was added to the supernatant. 15 µg protein was loaded on 3-12% Bis-Tris NativePAGE gels (Invitrogen) and run according to the manufacturer’s ...

I due tipi di allenamento che sto per descriverti sono molto importanti per runner di tutti i livelli, ma in pochissimi conoscono. In pratica si tratta di variare il ritmo del lento rendendolo ...

Manchester Institute of Biotechnology, School of Biological Sciences, Manchester Academic Health Sciences Centre, The University of Manchester, Princess Street, Manchester M1 7DN, U.K. Manchester ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Protein Kinase A (PKA) is regulated spatially ...

Recent advancements in single-molecule biophysics have been driven by breakthroughs in advanced fluorescence microscopy techniques and the development of next-generation organic fluorophores. These ...